Application

This Sheep Anti-Rabbit IgG Antibody, Cy5 conjugate is validated for use in Immunocytochemistry for the detection of Sheep Rabbit IgG.

Research Sub CategoryWhole Immunoglobulin Secondary Antibodies

Immunocytochemistry Analysis: Performance of this secondary antibody is validated (1.25 µg/mL) by indirect fluorescent labeling of H3K27me3 in HUVECs following a rabbit polyclonal parimary antibody (Cat. No. 07-449) in immunocytochemistry application.

Research CategorySecondary & Control Antibodies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.

Immunogen

Rabbit IgG

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein A purified

Purified sheep antibody Conjugate in PBS with 15 mg/ml BSA and 0.1 % sodium azide.

Quality

Evaluated by Immunocytochemistry in NIH/3T3 cells.

Immunocytochemistry Analysis: Performance of this secondary antibody is validated (1.25 µg/mL) by indirect fluorescent labeling of H3K27me3 in NIH/3T3 following a rabbit polyclonal parimary antibody (Cat. No. 07-449) in immunocytochemistry application.

Specificity

This antibody reacts with rabbit IgG and rabbit IgG (Fc). Minimal cross-reactivity detected with Human IgG and Rabbit IgM; little to no cross-reactivity detected with Mouse IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.